STUDY OF THE ALGAE COMPOSITION OF THE SALINE PARTS OF LAKE BALKHASH AND ISOLATION OF AXENIC CULTURES OF PHOTOTROPHIC MICROORGANISMS

Authors

DOI:

https://doi.org/10.26577/EJE86120267

Abstract

The presented work defines the species composition of the algal flora within the saline region of Lake Balkhash and involves isolating novel and promising cultures of microalgae and cyanobacteria, followed by examining their morphological, cultural, and physiological traits. The taken results indicated that the algal flora in water samples from the eastern coast of Balkhash is composed of diatoms (Bacillariophyta) at 40.33%, green algae (Chlorophyta) at 32%, cyanobacteria (Cyanophyta) at 19.33%, and euglenoids (Euglenophyta) at 8.33%. The specimens were classified into 4 divisions, 6 classes, 6 orders, 11 families, and 22 genera. Through the application of microbiological techniques, eight axenic pure cultures of microalgae and cyanobacteria were successfully isolated and determined, including Oscillatoria sp, Nostoc sp, Arthrospira sp., Anabaena sp, Chlorella vulgaris, Parachlorella kessleri, Dunaliella salina, and Navicula sp. Optimal cultivation conditions were determined for each strain. The isolated cyanobacterial cultures exhibited the highest growth rate on BG-11 and Zarrouk nutrient media at pH 7–9 and an optimal temperature of 28–32 °C. The isolated microalgae cultures showed effective growth on Tamia, Artaria, and BG-11 nutrient media at pH 6.5–7.5 and a temperature of 27–30 °C.The obtained pure cultures are notably valuable in industrial biotechnology, particularly for the development of biologically active supplements.

Keywords: algae, isolation of axenic pure cultures, microalgae and cyanobacteria cultures, saline lake.

Author Biographies

  • Aknur Toktybay , Al-Farabi Kazakh National University, Almaty, Kazakhstan

    Junior Research Fellow at the Scientific Research Institute of Problems of Biology and Biotechnology Ministry of Education and Science of the Republic of Kazakhstan

  • Damys Salauat, Al-Farabi Kazakh National University, Almaty, Kazakhstan

    PhD doctoral student of the 3nd year of the specialty "8D08401 – Fisheries and Industrial Fishing" Department of Biodiversity and Bioresources of Al-Farabi Kazakh National University

  • Kenzhegul Bolatkhan, Al-Farabi Kazakh National University, Almaty, Kazakhstan

    PhD, associate professor of the Department of biotechnology of the Al-Farabi Kazakh National University

  • Sandugash Sandybayeva, Al-Farabi Kazakh National University, Almaty, Kazakhstan

    PhD, Senior Lecturer at the Department of Biotechnology of Al-Farabi Kazakh National University

  • Gulnaz Akhmetova, Al-Farabi Kazakh National University, Almaty, Kazakhstan

    Senior Lecturer at the Department of Biotechnology of Al-Farabi Kazakh National University

  • Asem Kurmetkhan, Al-Farabi Kazakh National University, Almaty, Kazakhstan

    Fellow at the Scientific Research Institute of Problems of Biology and Biotechnology Ministry of Education and Science of the Republic of Kazakhstan

  • Sanzhar Sanatov, Al-Farabi Kazakh National University, Almaty, Kazakhstan

    1st-year Master’s student in Environmental Bioengineering at Al-Farabi Kazakh National University

  • Bolatkhan Zayadan, Al-Farabi Kazakh National University, Almaty, Kazakhstan

    Doctor of Biological Sciences, Professor of Biotechnology Department of al-Farabi Kazakh National University, Academician of the National Academy of Sciences of the Republic of Kazakhstan

  • Pavel Hrouzek , Institute of Microbiology of the Czech Academy of Sciences, Center Algatech, Trebon, Czech Republic

    Ph.D. Head of the laboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center Algatech

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Published

2026-03-31

Issue

Vol. 86 No. 1 (2026): Eurasian Journal of Ecology

Section

CURRENT PROBLEMS OF CONSERVATION OF BIOLOGICAL DIVERSITY

How to Cite

STUDY OF THE ALGAE COMPOSITION OF THE SALINE PARTS OF LAKE BALKHASH AND ISOLATION OF AXENIC CULTURES OF PHOTOTROPHIC MICROORGANISMS. (2026). Eurasian Journal of Ecology, 86(1). https://doi.org/10.26577/EJE86120267