Comparative analysis of methods for the concentration of aqueous samples containing viral material.

Abstract

The main characteristic of any ecosystem is the relationship of its components : the biotope and biocenosis, so any change in one of them will inevitably change the other. As indicators of ecosystem can use biometric indicators that allow early detection of adverse environmental impacts and take timely measures to limit anthropogenic pressures on ecosystems long before the emergence of critical , irreversible situation . In turn, the study of the ecological status of aquatic ecosystems one of the main problems is the concentration of samples of biological material for obtaining biometric indicators. The aim of the present study is a comparative study of 5 basic methods of concentration of biological material from water samples for further use and viral nucleic acids: centrifugation, membrane filtration, ethanol precipitation , salting out salts , and polyethylene glycol dehydration . The studies found that the different levels of viral particles of the different embodiments may require concentration of the sample.