Experimental study of biochemical markers of Mangystau oil’ hepatotoxic action

  • L. Sutuyeva Казахский национальный университет имени аль-Фараби
  • А. Ondasynova Казахский национальный университет имени аль-Фараби
  • M. Suvorova Казахский национальный университет имени аль-Фараби
  • B. Abdullayeva Казахский национальный университет имени аль-Фараби
  • T. Shalakhmetova Казахский национальный университет имени аль-Фараби


The article is dedicated to investigation of crude oil experimental exposure of rats to reveal the biochemical markers of those xenobiotic toxic action. It was established, that experimental exposure of rats to crude oil
(Karakuduk oil field) for 8 days leads to immediate induction of detoxication enzymes accompanied by increased lipid peroxidation in rats liver.


1 Tolosa I., Mora S., Reza S. M., Villeneuve J. P., Bartocci J., Cattini C. Aliphatic and aromatic hydrocarbons in coastal caspian Sea sediments // Marine Pollution Bulletin. - 2004. – V. 48, № 1-2. – P. 44-60.
2 Mansuy D. The great diversity of reactions catalyzed by cycohromes P450 // Comp. Biochem. Physiol. C. – 1998. – Vol.121. – P. 5–14.
3 Стальная И.Д., Гаришвили Г.Г. Определение МДА с помощью тиобарбитуровой кислоты // В сб.: Современные методы в биохимии. - 1977. №3.– С. 66-68.
4 Herbig W.J, Pabst M.J, Jacoby W.B. Glutathione S-transferase, the firdt enzymatic step in mercapturic acid formation // J. Biol. Chem. -1974. № 249. – P. 7139–7147.
5 Pradnya S. Walawalkar, Pooja S. Serai, Iyer K.R. Isolation and catalytic competence of different animal liver microsomal fractions prepared by calcium-aggregation method // Indian journal of pharmaceutical
sciences. - 2006. – V. 68, № I. 2. – P. 262-265.
6 Guengerich F.P. Analysis and characterization of enzymes. In: Principles and methods of toxicology, Hayes A.W (eds). 3rd Edn., Raven Press, New York. – 1994. – P. 1259–1313.
7 International Agency for Research on Cancer (IARC). Monographs. Occupational Exposures in Petroleum Refining; Crude Oil and Major Petroleum Fuels, 1989, Vol.45.
8 Deer A.K., Henczova M., Banka L., Varanka Z., Nemcsok J. Effects of crude oil and oil fractions on the liver P450–dependent monooxygenase activities and antioxidant defence system of different freshwater fish
species // Acta. Biol. Hung. – 2010. – Vol.61. – P. 262–273.
9 Achuba F.I., Osakwe S.A. Petroleum induced free radical toxicity in African catfish (Clarias gariepinus) // Fish Physiol. Biochem. – 2003. – Vol.29. – P. 97–103.
10 Adedara I.A., Teberen R., Ebokaiwe A.P., Ehwerhemuepha T., Farombi E.O. Induction of oxidative stress in liver and kidney of rats exposed to Nigerian bonny light crude oil // Envi-ron. Toxicol. – 2011. – Doi:
11 Gad N.S. Oxidative stress and antioxidant enzymes in Oreochromis niloticus as biomarkers of exposure to crude oil pollution // IJESE. – 2011. – Vol.1. – P. 49–58.
12 Mahmoud K., Shalakhmetova T., Deraz Sh et al. Changes in hepatic antioxidant defense system with er injury development in rats with crude oil intoxication // J.Biol.Chem.Environ.Sci. -2011. - vol6., N4.-P.483
– 500.
How to Cite
SUTUYEVA, L. et al. Experimental study of biochemical markers of Mangystau oil’ hepatotoxic action. Eurasian Journal of Ecology, [S.l.], v. 38, n. 2/2, jan. 2016. ISSN 2617-7358. Available at: <https://bulletin-ecology.kaznu.kz/index.php/1-eco/article/view/365>. Date accessed: 14 july 2020.


rats; crude oil; intoxication; detoxication; antioxidant system.