Construction of recombinant yeast strains useful for fermentation of cellulose-containing material

  • S. M. Taipakova Казахский национальный университет им. аль-Фараби
  • I. T. Smekenov Казахский национальный университет им. аль-Фараби
  • A. K. Bissenbaev Научно-исследовательский институт проблем биологии и биотехнологии

Abstract

Through the use of a multicopy plasmid and genomic integration vector, new yeast strains with high-level expression of heterologous endo-β-1,4-glucanase of the fungus Aspergillus niger and the Lentinula edodes
cellobiohydrolase CEL7A were obtained. The vector carrying a selective marker gene resistant to geneticin (G418), a constitutive promoter and terminator of yeast gene glyceraldehyde 3-phosphate dehydrogenase
(GAPDH) was created for integration of genetic material into the chromosomal HO locus. Approaches used in this study have been shown to be perspective in the development of industrial strains for the fermentation
of cellulose-containing materials.

References

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Published
2016-01-14
How to Cite
TAIPAKOVA, S. M.; SMEKENOV, I. T.; BISSENBAEV, A. K.. Construction of recombinant yeast strains useful for fermentation of cellulose-containing material. Eurasian Journal of Ecology, [S.l.], v. 38, n. 2/2, jan. 2016. ISSN 2617-7358. Available at: <https://bulletin-ecology.kaznu.kz/index.php/1-eco/article/view/373>. Date accessed: 14 july 2020.
Section
ТЕОРЕТИЧЕСКИЕ И ПРИКЛАДНЫЕ АСПЕКТЫ ЭКОЛОГИИ,БИОЛОГИИ И БИОТЕХНОЛОГИИ

Keywords

cellobiohydrolase; endo-1,4-β-glucanase; Saccharomyces cerevisiae; gene expression; enzymatic activity; glycosylation.