ОТБОР И ХАРАКТЕРИСТИКА МНОГОФУНКЦИОНАЛЬНЫХ МИКРОБНЫХ ШТАММОВ ДЛЯ РАЗРАБОТКИ СТРАТЕГИЙ ФИТОРЕМЕДИАЦИИ

Рамза Бержанова; Тогжан Мукашева; Нургуль Бектилеуова; Гүлшара Абай; Елнур Сатыбалды; Райхан Сыдыкбекова

doi:10.26577/EJE86120261

Authors

Ramza Berzhanova Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0000-0001-9251-4452
Togzhan Mukasheva Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0000-0003-3275-7895
Nurgul Bektileuova Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0000-0003-1998-7071
Gulshara Abay LLP «BioClean», Almaty, Kazakhstan https://orcid.org/0000-0003-1666-8366
Elnur Satybaldy Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0009-0005-7859-2303
Raihan Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0000-0002-7924-4977

DOI:

https://doi.org/10.26577/EJE86120261

Abstract

Characterization of PGPM microorganisms is an important step in selecting strains as bioinoculants. In this study, the PGPM properties of 98 isolates recovered from soil, rhizosphere, and plant tissues grown in oil-contaminated soil were assessed. Of the 98 isolates tested, 21.4% exhibited proteolytic activity, 24.4% exhibited amylolytic activity, and 9.2% exhibited cellulolytic activity on agar media with specific substrates. After the initial screening, 20 isolates (N9, N11, MSM12, MSM3, MSM20, MSM21, MSM25, MSM20, V16, L5-2, L5-5, L5-6, L10-2, L10-6, L10-12, L10-10, KP5, KB9, KP10 and C10) demonstrating high double or triple hydrolytic activity were selected. The stepwise screening methodology allowed the selection of strains demonstrating more than one or several properties with PGPR properties that ensure plant growth such as: phytohormone production, nitrogen fixation, IAA secretion, production of iron chelating compounds, phosphate solubility and synthesizing hydrolytic enzymes. Therefore, multifunctional strains with PGPR properties can be used as the most promising inoculants to improve the efficiency of phytoremediation of oil-contaminated soils.

Keywords: IAA, bacteria that promote plant growth, siderophores, phytohormones, phosphates, nitrogen fixation, protease, cellulase, amylase.

Author Biographies

Ramza Berzhanova, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Associate Professor of the Department of Biotechnology of Al-Farabi Kazakh National University
Togzhan Mukasheva, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Professor of the Department of Biotechnology of Al-Farabi Kazakh National University
Nurgul Bektileuova, Al-Farabi Kazakh National University, Almaty, Kazakhstan

2nd - year doctoral student of the Department of Biotechnology of Al-Farabi Kazakh National University
Gulshara Abay, LLP «BioClean», Almaty, Kazakhstan

Senior Researcher at BioClean LLC
Elnur Satybaldy, Al-Farabi Kazakh National University, Almaty, Kazakhstan

2nd - year Master's student of the Department of Biotechnology of Al-Farabi Kazakh National University
Raihan, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Associate Professor of the Department of Biotechnology of Al-Farabi Kazakh National University