Construction of recombinant yeast strains useful for fermentation of cellulose-containing material

Abstract

Through the use of a multicopy plasmid and genomic integration vector, new yeast strains with high-level expression of heterologous endo-β-1,4-glucanase of the fungus Aspergillus niger and the Lentinula edodes cellobiohydrolase CEL7A were obtained. The vector carrying a selective marker gene resistant to geneticin (G418), a constitutive promoter and terminator of yeast gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was created for integration of genetic material into the chromosomal HO locus. Approaches used in this study have been shown to be perspective in the development of industrial strains for the fermentation of cellulose-containing materials.