Creating dihaploids winter wheat microspore culture method

Abstract

As a result of cytological monitoring it was found that on the basis of morphological observations mononuclear microspore developmental stage corresponds to the phase when the second leaf sheath be in the middle ear (anther dark green). The best option sterilization number 8, which provided the highest percentage of net microspores - 4.8% in the petri dish. The most optimal for the cultivation of winter wheat microspore is liquid W14 mf medium with the addition of Ficoll and maltose. Together in biotechnology laboratories Saaten Union Biotec Germany and Kazakhstan produced 1,325 regenerated plants, of which 1,153 green plants are 5 varieties of winter wheat in the laboratory of biotechnology Saaten Union Biotecand 172 plants in the laboratory of biotechnology KazNIIZR.