Creating dihaploids winter wheat microspore culture method

  • K. R. Urazaliev Казахский научно-исследовательский институт земледелия и растениеводства
  • J. M. Orsini Saaten-Union Biotec GmbH
  • A. M. Abekova Казахский научно-исследовательский институт земледелия и растениеводства
  • T. A. Bazylova Казахский научно-исследовательский институт земледелия и растениеводства
  • A. K. Daniayrova Казахский научно-исследовательский институт земледелия и растениеводства


As a result of cytological monitoring it was found that on the basis of morphological observations mononuclear microspore developmental stage corresponds to the phase when the second leaf sheath be in the middle ear (anther dark green). The best option sterilization number 8, which provided the highest percentage of net microspores - 4.8% in the petri dish. The most optimal for the cultivation of winter wheat microspore is liquid W14 mf medium with the addition of Ficoll and maltose. Together in biotechnology laboratories Saaten Union Biotec Germany and Kazakhstan produced 1,325 regenerated plants, of which 1,153 green plants are 5 varieties of winter wheat in the laboratory of biotechnology Saaten Union Biotecand 172 plants in the laboratory of biotechnology KazNIIZR.


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How to Cite
URAZALIEV, K. R. et al. Creating dihaploids winter wheat microspore culture method. Eurasian Journal of Ecology, [S.l.], v. 38, n. 2/2, jan. 2016. ISSN 2617-7358. Available at: <>. Date accessed: 14 july 2020.


haploid technology; isolated microspore culture; winter wheat; culture medium