Evaluation of biotechnological potential of thermotolerant microorganisms for oil degradation

  • Айткельдиева С.А., Файзулина Э.Р., Ауэзова О.Н., Спанкулова Г.А., Татаркина Л.Г., Алимбетова А.В. LLC “Research and Production Center for Microbiology and Virology”, Kazakhstan, Almaty,

Abstract

The problem of pollution of natural ecosystems with oil and oil products is extremely acute in manycountries of the world, including Kazakhstan. A significant part of oil-contaminated sites is located inregions with a hot climate, and in such countries studies are underway to isolate thermotolerant oildegrading microorganisms with a view to their further use for remediation of oil-contaminated territories.The circle of such microorganisms is quite wide and diverse.The effectiveness of the method of bioremediation of oil-polluted ecosystems largely depends onbiotic and abiotic factors, for example, salinity, temperature, ability to produce surfactants, pH, etc.Earlier from the soils of Western Kazakhstan, we isolated and identified thermotolerant oil-oxidizing microorganisms capable of oxidizing oil hydrocarbons in a wide temperature range. The aim of this workwas to study the ability of active thermotolerant oil-oxidizing microorganisms to degrade oil at different concentrations of mineral salts (Cl-, SO42-) and to determine the emulsifying activity to assess theirbiotechnological potential.It was established that all selected strains are halotolerant. Moreover, the temperature factor influenced the degree of oil degradation in the presence of mineral salts. The highest percentage of oildegradation was at 35°C. At this temperature, strains K-3, 4/5, P1-35-14 showed high activity at allstudied NaCl concentrations (3, 5 and 10%) – 73.6-85.9%, 62.7-91.1 % and 73.6-75.7%, respectively.At 40°C, the strain IP-40-4 showed the highest activity – 41.4-51.2%. At 50°C, the destruction abilityof the studied cultures was approximately at the same level at all NaCl concentrations. The cultivationof selected strains with a high content of MgSO4 in the medium showed that the K-3 strain was the moststable, which remained highly active at all temperatures.It was shown that active strains of thermotolerant oil-oxidizing microorganisms had emulsifying activity. High values of emulsifying activity are observed at a temperature of 35°C, and with a subsequentincrease in temperature, the formation of surfactants decreased. At 35°C, strain P2-35-9 had the greatestemulsifying activity, and at 40°C and 50°C – strain K-3.Key words: bioremediation, thermotolerant hydrocarbon-oxidizing bacteria, salinity, halotolerance,emulsifying activity.
Published
2020-06-24
How to Cite
ТАТАРКИНА Л.Г., АЛИМБЕТОВА А.В., Айткельдиева С.А., Файзулина Э.Р., Ауэзова О.Н., Спанкулова Г.А.,. Evaluation of biotechnological potential of thermotolerant microorganisms for oil degradation. Eurasian Journal of Ecology, [S.l.], v. 2, n. 63, p. 4-14, june 2020. ISSN 2617-7358. Available at: <https://bulletin-ecology.kaznu.kz/index.php/1-eco/article/view/1030>. Date accessed: 21 sep. 2020. doi: https://doi.org/10.26577/EJE.2020.v63.i2.01.
Section
ВОЗДЕЙСТВИЕ НА ОКРУЖАЮЩУЮ СРЕДУ АНТРОПОГЕННЫХ ФАКТОРОВ И ЗАЩИТА ОКРУЖАЮЩЕЙ СРЕДЫ