Comparative analysis of methods for the concentration of aqueous samples containing viral material.
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Keywords:
concentration, viruses, nucleic acid, polymerase chain reaction, the infectious dose,Abstract
The main characteristic of any ecosystem is the relationship of its components : the biotope and biocenosis, so any change in one of them will inevitably change the other. As indicators of ecosystem can use biometric indicators that allow early detection of adverse environmental impacts and take timely measures to limit anthropogenic pressures on ecosystems long before the emergence of critical , irreversible situation . In turn, the study of the ecological status of aquatic ecosystems one of the main problems is the concentration of samples of biological material for obtaining biometric indicators. The aim of the present study is a comparative study of 5 basic methods of concentration of biological material from water samples for further use and viral nucleic acids: centrifugation, membrane filtration, ethanol precipitation , salting out salts , and polyethylene glycol dehydration . The studies found that the different levels of viral particles of the different embodiments may require concentration of the sample.References
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2 Veeraraghavan N., Sreevalsan M. Evaluation of Some Methods of Concentration and Purification of Influenza Virus
Bull. World Health Organization. – 1961. – 24. – 695-702.
3 Reed L., Muench H. A simple method of estimating fifty percent endpints // Amer.J. Hyg. – 1938. – Vol. 27. – P.
493-497.
4 A Comparison of Methods for Counting Viruses in Aquatic Systems Yvan Bettarel, Christian Amblard, Henri Laveran Applied and Environmental Microbiology. – 2000. – Р. 2283–2289.
5 Урбах В.Ю. Статистический анализ в биологических и медицинских исследованиях. – М.: Медицина, 1975. –296 с.
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Published
2016-01-14
How to Cite
Alexyuk, M. S. (2016). Comparative analysis of methods for the concentration of aqueous samples containing viral material. Eurasian Journal of Ecology, 41(2). Retrieved from https://bulletin-ecology.kaznu.kz/index.php/1-eco/article/view/194
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Section
ENVIRONMENTAL IMPACT OF ANTHROPOGENIC FACTORS AND ENVIRONMENTAL PROTECTION
