Zayadan B., Usserbayeva A., Bolatkhan K., Akmukhanova N., Kossalbayev B., Baizhigitova A., Los D. Screening of isolated and collection strains of cyanobacteria on productivity for determining their biotechnological potential

al-Farabi Kazakh National University, Kazakhstan, Almaty

Abstract

Cyanobacteria produce a wide range of metabolites, such as proteins, carbohydrates, carotenoids,
vitamins and lipids which can be used as potential food sources for human and animals, in pharmaceutical and cosmetic industries and also as energy source. It is known that the technology of using cyanobacteria as fuel raw materials makes one of the central places among the approaches of modern alternative
energy. To date, the possibility of using cyanobacteria in wastewater treatment with the further use of
biomass for obtaining biodiesel fuel is being considered. For their application in biotechnology the strain
screening for producers is needed. Prospectivity of types and species for use in biotechnology is firstly
determined by their productivity. The article presents data of screening of collection and isolated strains
of cyanobacteria: Cyanobacterium sp. B-1200, Cyanobacterium aponium IPPAS B-1201, Synechococcus
elongatus 7942, Anabaena variabilis R-I-5 and Nostoc calsicola RI-3. Comparative analysis of constant
fluorescence indices, cell growth rate and accumulation of dry weight in cyanobacteria was carried
out. Thus, as a result of investigations, the greatest optical density was noted in Anabaena variabilis
R-I-5 strains (1,191) and Cyanobacterium sp. B-1200 (1,281). Fluorescence indices also confirmed the
high photosynthetic activity of cyanobacteria Cyanobacterium sp. B-1200 (26101 rel.un.) and Anabaena
variabilis R-I-5 (25054 rel.un). Also, investigated strains Anabaena variabilis R-I-5 and Cyanobacterium
aponium IPPAS B-1201 were characterized by relatively high biomass accumulation – 1,19 g/l и 1,36
g/l correspondingly. According to obtained data, it was established that cyanobacteria Cyanobacterium
sp. B-1200 and Anabaena variabilis R-I-5 have a relatively high values of growth rate, fluorescence and
biomass yield which determine their high productivity. Thus, in the result of screening strains Cyanobacterium stanieri B-1 and Anabaena variabilis R-I-5 were selected for further investigations of their
physiological and biochemical properties with the purpose to determine potential producers of valuable
metabolites for biotechnology.
Key words: screening, cyanobacteria, biomass, fluorescence, biotechnological potential