Associated risks of lung cancer with radon emanation

Abstract

Lung cancer occupies an exceptional position. It is a rare example
of a malignant disease with firmly established and delineated etiological
factors. In fact, in most cases, the occurrence of the lung cancer can be
associated with smoking; Moreover, this tumor can be connected with
other carcinogenic agents in the inhaled air such as radon gas. Radon is
the second leading cause of lung cancer. The aim of our work was to
study the C-KIT protein expression by immunohistochemical method and
identification of germ-line mutations in lung cancer patients who live in
Almaty, where the radon level exceeds its norm. The methods chosen to
carry out this study were a polymerase chain reaction (PCR) with a subsequent analysis of restriction fragment length polymorphism (RFLP) and
immunohistochimical analysis (IHC). The object of research were blood
samples and biopsies obtained from lung cancer patients who are receiving treatment at the Almaty Oncology Center and living in Almaty. Our
studies didn’t reveal any mutations in the C-KIT gene, but they showed
that 34.09% of lung cancer patients have overexpression in a tumor tissue.
It testifies to the fact that disorders occurred in the cells of the tumor tissue,
that are not inherited. Based on our observations, we arrived at conclusion
that a high radon emanation has caused disorder in the C-KIT gene, which
led to its overexpression.
Key words: C-KIT, Expression, radon, IHC, lung cancer.